Anti Fibrinogen (human)
Mouse monoclonal antibody
Cat.No. HYB 051-04
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Fibrinogen, the precursor of fibrin, is the coagulable protein in the blood plasma of vertebrates. Fibrinogen consists as a dimer of 3 pairs of non-identical chains Aa (Mr= 66,800), Bb (Mr= 52,000) and g (Mr=46,500) cross-linked by disulfide bonds in their N-terminal segments. The molecule has 2 terminal D domains and one central E domain, all three domains are separated when fibrinogen is degradated by plasmin. MW is 340 kDa (1). Concentration in human plasma is 2-3 mg/ml (2).
Fibrinogen isolated from human plasma
HYB 051-04 has specificity for human fibrinogen
Epitope specificity differs from that of HYB 051-07 as determined by inhibition ELISA.
HYB 051-04 reacts strongly with fibrinogen. Strong reaction is seen in ELISA with factor VIII HP directly coated onto the microtiterwell, also when tested in sandwich ELISA in combination with a polyclonal antibody against fibrinogen (eg. DAKO A 080). In Western blotting after SDS-PAGE, HYB 051-04 reacts with fibrinogen in non-reduced form only.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:8000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Doolittle RF (1977) Structure and function of fibrinogen. Horiz Biochem Biophys 3:164-191.
2. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.
